Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type enzyme with and without bound inhibitor, hanging drop vapour diffusion method, 4°C, 18 mg/ml protein solution containing 10 mM HEPES, pH 7.5, 150 mM KCl, 1 mM 1,4-dithio-D,L-threitol, with or without 1 mM 4-hydroxyphenacyl-CoA, plus precipitant solution containing 17-20% PEG 3400, 100 mM MOPS, pH 7.0, and 200 mM LiCl, X-ray diffraction structure determination at 2.6 A resolution, and analysis | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybenzyl-CoA | - |
Arthrobacter sp. | |
4-hydroxyphenacyl-CoA | binding mechanism | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Hydroxybenzoyl-CoA + H2O | Arthrobacter sp. | enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria | 4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | Arthrobacter sp. SU / DSM 20407 | enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria | 4-Hydroxybenzoate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
- |
- |
Arthrobacter sp. SU / DSM 20407 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-Hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA | active site structure and ligand binding mechanism, overview | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-Hydroxybenzoyl-CoA + H2O | - |
Arthrobacter sp. | 4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria | Arthrobacter sp. | 4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | - |
Arthrobacter sp. SU / DSM 20407 | 4-Hydroxybenzoate + CoA | - |
? | |
4-Hydroxybenzoyl-CoA + H2O | enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria | Arthrobacter sp. SU / DSM 20407 | 4-Hydroxybenzoate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | dimer of dimers, structure analysis | Arthrobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
Hydrolase, 4-hydroxybenzoyl coenzyme A | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arthrobacter sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000003 | - |
4-hydroxyphenacyl-CoA | pH 7.5, 25°C | Arthrobacter sp. | |
0.0006 | - |
4-hydroxybenzyl-CoA | pH 7.5, 25°C | Arthrobacter sp. |